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Equipment: TECAN SPARK 10M
Technique: spectrophotometer
Responsible: Carolina Marques /

Description: The TECAN SPARK 10M is a multifunctional microplate reader equipped with a high-speed monochromator (HSM), allowing full absorbance spectrum scan in less than 5 minutes. The equipment accepts 1 – 384 wells plate format and can be used to perform kinetics measurements autonomously. The SPARK is equipped with the SparkControl software for reader control and data reduction.

Specifications: 

  • Spectral Range: 200 – 1000 nm    
  • OD range: 0 – 4 OD    
  • Scan Speed 200-1000 nm: ≤ 5 sec    
  • Wavelength accuracy: < 0.3 nm    
  • Wavelength reproducibility: ≤ 0.3 nm    
  • Wavelength ratio accuracy 260/230: < 0.08    
  • Wavelength ratio accuracy 260/280: < 0.07    
  • OD precision @ 260 nm: ≤ 0.2%    
  • OD accuracy @ 260 nm: ≤ 0.5%    
  • Limit of detection (DNA): < 1 ng/µl

Link for additional information:
https://www.cenimat.fct.unl.pt/lab-facilities/characterization-lab/micro-plate-reader
https://lifesciences.tecan.com/multimode-plate-reader
https://content.ilabsolutions.com/wp-content/uploads/2018/09/Tecan-SPark-10M_V1_1.pdf